Bethyl Laboratories
Rabbit anti-Calreticulin Antibody Affinity Purified
Primary
Catalog #A301-130A
TargetCalreticulin
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
Calreticulin is identified as a multifunctional protein that acts as a lectin chaperone and calcium binding protein. Calreticulin is found in the lumen of the endoplasmic reticulum and nucleus. In the nucleus, Calreticulin appears to function as a coregulator of nuclear hormone receptors. Increased autoantibody titers against calreticulin are associated with systemic lupus.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 367 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetCalreticulin
Antigen Species
Human
Gene ID811
Gene Symbol
CALR
Gene Name
calreticulin
Uniprot IDP27797
Protein Name
Calreticulin
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Calreticulin immobilized on solid support.
The epitope recognized by A301-130A maps to a region between residue 367 and 417 of human calreticulin (Sicca syndrome antigen A ) using the numbering given in entry NP_004334.1 (GeneID 811).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-130A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-130A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-130AOpen
Citations / Publications
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