Bethyl Laboratories
Rabbit anti-PAF49 Antibody Affinity Purified
Primary
Catalog #A301-294A
TargetPAF49
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
PAF49 is subunit of RNA polymerase I. PAF49/CAST has been shown to interact with activator upstream binding factor (UBF) and plays an important role in rDNA transcription.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 250 and 300
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPAF49
Antigen Species
Human
Gene ID10849
Gene Symbol
CD3EAP
Gene Name
CD3e molecule associated protein
Uniprot IDO15446
Protein Name
DNA-directed RNA polymerase I subunit RPA34
Alternate Names
A34.5 • antisense to ERCC-1 protein • ASE1 • ASE-1 • CAST • CD3e antigen, epsilon polypeptide associated protein • CD3e molecule, epsilon associated protein • CD3E-associated protein • CD3-epsilon-associated protein • DNA-directed RNA polymerase I subunit RPA34 • PAF49 • RNA polymerase I-associated factor PAF49
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PAF49 immobilized on solid support.
The epitope recognized by A301-294A maps to a region between residue 225 and 275 of human RNA polymerase I-associated factor PAF49 (CD3-epsilon-associated protein) using the numbering given in entry NP_036231.1 (GeneID 10849).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-294A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-294A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-294AOpen
Citations / Publications
Services
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