Bethyl Laboratories
Rabbit anti-Rtf1 Antibody Affinity Purified
Primary
Catalog #A301-329A
TargetRtf1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
In yeast, Rtf1 is a component of the Paf1/RNA polymerase II complex. The Paf1 complex interacts with RNA polymerase II and functions to modify chromatin during transcription. Rtf1 appears to be important for histone H2B ubiquitination by Rad6 and for methylation of histone H3 catalyzed by the Set1/COMPASS complex that functions during transcription elongation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 660 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetRtf1
Antigen Species
Human
Gene ID23168
Gene Symbol
RTF1
Gene Name
RTF1 homolog, Paf1/RNA polymerase II complex component
Uniprot IDQ92541
Protein Name
RNA polymerase-associated protein RTF1 homolog
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Rtf1 immobilized on solid support.
The epitope recognized by A301-329A maps to the C-terminus of human Restores TBP (TATA box-binding protein) Function-1 using the numbering given in entry EAW92492.1 (GeneID 23168).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-329A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-329A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-329AOpen
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