Bethyl Laboratories
Rabbit anti-AKAP1 Antibody Affinity Purified
Primary
Catalog #A301-379A
TargetAKAP1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
Image 1 of 2
local_shipping
Order within 16 hours and 32 minutes for shipment by today
Product Description
AKAP1 (A-kinase anchor protein 1) was isolated as a protein that interacts with the regulatory subunit of protein kinase A (PKA) as well as the catalytic subunit of protein phosphatase 1 (PPI). AKAP1 plays a role in directing the subcellular localization of PKA to the cytoplasmic face of the outer mitochondrial membrane.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 375 and 425
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetAKAP1
Antigen Species
Human
Gene ID8165
Gene Symbol
AKAP1
Gene Name
A-kinase anchoring protein 1
Uniprot IDQ92667
Protein Name
A-kinase anchor protein 1, mitochondrial
Alternate Names
A kinase (PRKA) anchor protein 1 • AKAP • AKAP 149 • AKAP121 • AKAP149 • AKAP84 • A-kinase anchor protein 1, mitochondrial • A-kinase anchor protein 149 kDa • D-AKAP1 • D-AKAP-1 • Dual specificity A-kinase-anchoring protein 1 • dual-specificity A-kinase anchoring protein 1 • PPP1R43 • PRKA1 • protein kinase A anchoring protein 1 • protein kinase A1 • Protein kinase A-anchoring protein 1 • protein phosphatase 1, regulatory subunit 43 • SAKAP84 • S-AKAP84 • spermatid A-kinase anchor protein 84 • TDRD17 • testicular secretory protein Li 5 • tudor domain containing 17
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to AKAP1 immobilized on solid support.
The epitope recognized by A301-379A maps to a region between residue 375 and 425 of human A kinase (PRKA) anchor protein 1 using the numbering given in entry NP_003479.1 (GeneID 8165).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-379A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-379A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-379AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1