Bethyl Laboratories
Rabbit anti-PABP4 Antibody Affinity Purified
Primary
Catalog #A301-466A
TargetPABP4
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
PABP4 (poly(A)-binding protein 4), also known as iPABP (inducible poly(A)-binding protein) was originally identified in a search for activation-induced genes in T-cells. It is one of five poly(A)-binding proteins encoded by independent genes whose products play roles in various aspects of mRNA processing. The PABP genes encode one nuclear and four cytoplasmic PABP proteins. PABP2/PABP (PABPN1) is the observed nuclear PABP. The cytoplasmic PABPs include PABP1 (PABPC1), PABP3 (PABPC3), PABP4/iPABP (PABPC4), and x-linked PABP5 (PABPC5). The cytoplasmic PABPs bear four RNA-recognition motifs (RRMs) that participate in binding poly (A). In T-cells, PABP4 is proposed to play a role in stabilizing labile mRNA.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 525 and 575
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetPABP4
Antigen Species
Human
Gene ID8761
Gene Symbol
PABPC4
Gene Name
poly(A) binding protein cytoplasmic 4
Uniprot IDQ13310
Protein Name
Polyadenylate-binding protein 4
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PABP4 immobilized on solid support.
The epitope recognized by A301-466A maps to a region between residue 525 and 575 of human Poly(A)-binding protein 4 using the numbering given in entry NP_003810.1 (GeneID 8761).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-466A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-466A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-466AOpen
Citations / Publications
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