Bethyl Laboratories
Rabbit anti-SART3/TIP110 Antibody Affinity Purified
Primary
Catalog #A301-521A
TargetSART3/TIP110
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
SART3 (squamous cell carcinoma antigen recognized by T cells 3) was identified as an antigenic peptide expressed in esophageal cancer cells that is recognized by cytotoxic T lymphocytes. Originally, in a search for a U6 small nuclear RNA capping enzyme, SART3 was identified as a 110kDa nuclear RNA-binding protein. Later, by database searching, SART 3 was determined to also be the human ortholog of the yeast Prp24 snRNP recycling factor that functions in the reassembly of the U4/U6 snRNP during the recycling phase of the splicesome cycle. Recently, SART3-derived peptides have been investigated as candidates for peptide-based immunotherapy in prostate cancer patients due to their ability to induce cytotoxic T lymphocyte activity against prostate cancer cells.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 600 and 650
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSART3/TIP110
Antigen Species
Human
Gene ID9733
Gene Symbol
SART3
Gene Name
squamous cell carcinoma antigen recognized by T-cells 3
Uniprot IDQ15020
Protein Name
Squamous cell carcinoma antigen recognized by T-cells 3
Alternate Names
DSAP1 • HIV-1 Tat-interacting protein of 110kDa • hSART-3 • P100 • p110 • p110 nuclear RNA-binding protein • p110(nrb) • RP11-13G14 • SART-3 • squamous cell carcinoma antigen recognized by T cells 3 • squamous cell carcinoma antigen recognized by T-cells 3 • tat-interacting protein of 110 kDa • TIP110
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SART3/TIP110 immobilized on solid support.
The epitope recognized by A301-521A maps to a region between residue 600 and 650 of human squamous cell carcinoma antigen recognized by T-cells 3 (Tat-interacting protein of 110 kDa) using the numbering given in entry NP_055521.1 (GeneID 9733).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-521A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-521A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-521AOpen
Citations / Publications
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