Bethyl Laboratories
Rabbit anti-UTP18/WDR50 Antibody Affinity Purified
Primary
Catalog #A301-551A
TargetUTP18/WDR50
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
UTP18/WDR50 is a component of the small-subunit (SSU) processome, a large ribonucleoprotein complex that participates in ribosomal RNA processing and ribosome assembly. UTP18/WDR50 is a member of the WD repeat family. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 100 and 150
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetUTP18/WDR50
Antigen Species
Human
Gene ID51096
Gene Symbol
UTP18
Gene Name
UTP18, small subunit processome component
Uniprot IDQ9Y5J1
Protein Name
U3 small nucleolar RNA-associated protein 18 homolog
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to UTP18/WDR50 immobilized on solid support.
The epitope recognized by A301-551A maps to a region between residue 75 and 125 of human small subunit (SSU) processome component UTP18 (WD repeat domain 50) using the numbering given in entry NP_057085.2 (GeneID 51096).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-551A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-551A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-551AOpen
Citations / Publications
Services
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