Bethyl Laboratories
Rabbit anti-METTL3/MT-A70 Antibody Affinity Purified
Primary
Catalog #A301-568A
TargetMETTL3/MT-A70
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
METTL3/MT-A70 is a subunit of the N6-methyltransferase that specifically methylates adenines in pre-mRNAs.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 530 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMETTL3/MT-A70
Antigen Species
Human
Gene ID56339
Gene Symbol
METTL3
Gene Name
methyltransferase like 3
Uniprot IDQ86U44
Protein Name
N6-adenosine-methyltransferase 70 kDa subunit
Alternate Names
adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase • hMETTL3 • IME4 • M6A • methyltransferase-like protein 3 • mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase • mRNA m(6)A methyltransferase • MT-A70 • N6-adenosine-methyltransferase 70 kDa subunit • N6-adenosine-methyltransferase catalytic subunit • Spo8
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to METTL3/MT-A70 immobilized on solid support.
The epitope recognized by A301-568A maps to a region between residue 530 and 580 of human methyltransferase like 3 (N6-adenosine-methyltransferase 70 kDa subunit) using the numbering given in entry NP_062826.2 (GeneID 56339).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-568A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-568A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-568AOpen
Citations / Publications
Services
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