Bethyl Laboratories
Rabbit anti-GRWD1/WDR28 Antibody Affinity Purified
Primary
Catalog #A301-576A
TargetGRWD1/WDR28
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
GRWD1/WDR28 contains five WD repeats and is a member of the WD family of proteins. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. WD proteins are involved in a variety of cellular processes. GRWD1/WDR28 is proposed to play a role in ribosome biogenesis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetGRWD1/WDR28
Antigen Species
Human
Gene ID83743
Gene Symbol
GRWD1
Gene Name
glutamate rich WD repeat containing 1
Uniprot IDQ9BQ67
Protein Name
Glutamate-rich WD repeat-containing protein 1
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to GRWD1/WDR28 immobilized on solid support.
The epitope recognized by A301-576A maps to a region between residue 1 and 50 of human glutamate-rich WD repeat containing 1 (WD repeat domain 28) using the numbering given in entry NP_113673.2 (GeneID 83743).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-576A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-576A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-576AOpen
Citations / Publications
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