Bethyl Laboratories
Rabbit anti-CDC5L Antibody Affinity Purified
Primary
Catalog #A301-681A
TargetCDC5L
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
CDC5L (cell division cycle 5-like protein) has been identified as a component of the human splicesome and is important to pre-mRNA splicing as well as cell cycle progression.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 100 and 150
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCDC5L
Antigen Species
Human
Gene ID988
Gene Symbol
CDC5L
Gene Name
cell division cycle 5 like
Uniprot IDQ99459
Protein Name
Cell division cycle 5-like protein
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CDC5L immobilized on solid support.
The epitope recognized by A301-681A maps to a region between residue 100 and 150 of human cell division cycle 5 (CDC5) -like using the numbering given in entry NP_001244.1 (GeneID988).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-681A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-681A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-681AOpen
Citations / Publications
Services
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