Bethyl Laboratories
Rabbit anti-eEF2 Kinase Antibody Affinity Purified
Primary
Catalog #A301-686A
TargeteEF2 Kinase
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
Eukaryotic elongation factor-2 kinase (eEF2 kinase) is a calcium- and calmodulin-dependent kinase that phosphorylates eukaryotic elongation factor 2 (eEF2), a non-ribosomal factor that mediates ribosomal translocation. eEF2 is regulated by MAP kinase signaling and the mTOR signaling pathway. eEF2 is phosphorylated in response to stimuli that increases energy demand or reduces its supply. Phosphorylation inhibits eEF2 activity to conserve energy.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargeteEF2 Kinase
Antigen Species
Human
Gene ID29904
Gene Symbol
EEF2K
Gene Name
eukaryotic elongation factor 2 kinase
Uniprot IDO00418
Protein Name
Eukaryotic elongation factor 2 kinase
Alternate Names
alternative protein EEF2K • Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase • calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase • calmodulin-dependent protein kinase III • CaMKIII • eEF-2 kinase • eEF-2K • elongation factor-2 kinase • eukaroytic elongation factor 2 kinase • eukaryotic elongation factor 2 kinase • HSU93850
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to eEF2 Kinase immobilized on solid support.
The epitope recognized by A301-686A maps to a region between residue 1 and 50 of human calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase using the numbering given in entry NP_037434.1 (GeneID 29904).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-686A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-686A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-686AOpen
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