Bethyl Laboratories
Rabbit anti-eEF2 Antibody Affinity Purified
Primary
Catalog #A301-688A
TargeteEF2
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, WB
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Product Description
Eukaryotic translation elongation factor 2 (eEF2) is a non-ribosomal protein that mediates ribosomal translocation and catalyzes peptide elongation in the presence of GTP. eEF2 is a substrate of the eEF2 kinase. Phosphorylation of eEF2 in response to increased energy demand or reduction in supply inhibits eEF2 activity for the conservation of energy.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 808 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargeteEF2
Antigen Species
Human
Gene ID1938
Gene Symbol
EEF2
Gene Name
eukaryotic translation elongation factor 2
Uniprot IDP13639
Protein Name
Elongation factor 2
Alternate Names
Product Information
Applications
IHC, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to eEF2 immobilized on solid support.
The epitope recognized by A301-688A maps to a region between residue 808 and 858 of human eukaryotic translation elongation factor 2 using the numbering given in entry NP_001952.1 (GeneID 1938).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:200 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-688A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-688A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-688AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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