Bethyl Laboratories
Rabbit anti-eIF5B/IF2 Antibody Affinity Purified
Primary
Catalog #A301-745A
TargeteIF5B/IF2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
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Product Description
Eukaryotic initiation factor 5 subunit B (eIF5B) is the eukaryotic ortholog of the bacterial IF2 initiation factor. It is one of two essential GTPases required for translation initiation. eIF5B plays a role in facilitiating 60S and 40S ribosomal subunit joining to form the 80S ribosome in the final step of translation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 450 and 500
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargeteIF5B/IF2
Antigen Species
Human
Gene ID9669
Gene Symbol
EIF5B
Gene Name
eukaryotic translation initiation factor 5B
Uniprot IDO60841
Protein Name
Eukaryotic translation initiation factor 5B
Product Information
Applications
WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to eIF5B/IF2 immobilized on solid support.
The epitope recognized by A301-745A maps to a region between residue 450 and 500 of human eukaryotic translation initiation factor 5B using the numbering given in entry NP_056988.3 (GeneID 9669).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-745A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-745A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-745AOpen
Citations / Publications
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