Bethyl Laboratories
Rabbit anti-eIF3H/EIF3S3 Antibody Affinity Purified
Primary
Catalog #A301-754A
TargeteIF3H/EIF3S3
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
Eukaryotic initiation factor 3 subunit H (eIF3H) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5’-end of cellular mRNAs near the cap structure (m7GpppN). eIF3H is a non-conserved subunit and part of the functional core of eIF3. It may function to stabilize the eIF3 complex and stimulate protein synthesis. Many cancer cell lines have been shown to express high levels of eIF3H, and it may have an oncogenic role in colorectal cancer susceptibility.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 302 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargeteIF3H/EIF3S3
Antigen Species
Human
Gene ID8667
Gene Symbol
EIF3H
Gene Name
eukaryotic translation initiation factor 3 subunit H
Uniprot IDO15372
Protein Name
Eukaryotic translation initiation factor 3 subunit H
Alternate Names
eIF3 p40 subunit • eIF-3-gamma • eIF3-gamma • eIF3h • eIF3-p40 • EIF3S3 • eukaryotic translation initiation factor 3 subunit 3 • eukaryotic translation initiation factor 3 subunit H • eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD) • eukaryotic translation initiation factor 3, subunit 3 (gamma, 40kD) • eukaryotic translation initiation factor 3, subunit 3 gamma, 40kDa
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to eIF3H/EIF3S3 immobilized on solid support.
The epitope recognized by A301-754A maps to a region between residue 302 and 352 of human eukaryotic translation initiation factor 3 subunit H (eukaryotic translation initiation factor 3, subunit 2) using the numbering given in entry NP_003747.1 (GeneID 8667).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-754A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-754A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-754AOpen
Citations / Publications
Services
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