Bethyl Laboratories
Rabbit anti-eIF3B/EIF3S9 Antibody Affinity Purified
Primary
Catalog #A301-761A
TargeteIF3B/EIF3S9
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
Eukaryotic initiation factor 3 subunit B (eIF3B) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5’-end of cellular mRNAs near the cap structure (m7GpppN). eIF3B is a conserved subunit and part of the functional core of eIF3. It bears a non-canonical RNA recognition motif (RRM) and interacts directly with the eIF3S1 subunit.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 100 and 150
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargeteIF3B/EIF3S9
Antigen Species
Human
Gene ID8662
Gene Symbol
EIF3B
Gene Name
eukaryotic translation initiation factor 3 subunit B
Uniprot IDP55884
Protein Name
Eukaryotic translation initiation factor 3 subunit B
Alternate Names
eIF3 p110 • eIF3 p116 • eIF3b • eIF-3-eta • EIF3-ETA • EIF3-P110 • EIF3-P116 • EIF3S9 • eukaryotic translation initiation factor 3 subunit 9 • eukaryotic translation initiation factor 3 subunit B • eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD) • eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa • hPrt1 • PRT1 • prt1 homolog
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to eIF3B/EIF3S9 immobilized on solid support.
The epitope recognized by A301-761A maps to a region between residue 100 and 150 of human eukaryotic translation initiation factor 3, subunit B (eukaryotic translation initiation factor 3, subunit 9) using the numbering given in entry NP_003742.2 (GeneID 8662).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-761A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-761A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-761AOpen
Citations / Publications
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