Bethyl Laboratories
Rabbit anti-NUP35 Antibody Affinity Purified
Primary
Catalog #A301-781A
TargetNUP35
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
Image 1 of 3
local_shipping
Order within 7 hours and 9 minutes for shipment by today
Product Description
The nuclear pore complex (NPC) is a structure that spans the nuclear envelope and provides a conduit between the nucleus and cytoplasm for the nucleocytoplasmic transport of macromolecules. The main components of the NPC are nucleoporins. There are about 30 different human Nups that have been identified. Human Nup35 is the 35 kDa ortholog of the yeast Nup53p. Nup35 is also referred to as Nup53 or vertebrate Nup53. Nup35 is a component of a NPC subcomplex that contains Nup35, Nup93, Nup155, and Nup205.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 276 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetNUP35
Antigen Species
Human
Gene ID129401
Gene Symbol
NUP35
Gene Name
nucleoporin 35
Uniprot IDQ8NFH5
Protein Name
Nucleoporin NUP53
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NUP35 immobilized on solid support.
The epitope recognized by A301-781A maps to a region between residue 276 and 376 of human nucleoporin 35kDa using the numbering given in entry NP_612142.2 (GeneID 129401).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-781A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-781A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-781AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1