Bethyl Laboratories
Rabbit anti-NUPL Antibody Affinity Purified
Primary
Catalog #A301-792A
TargetNUPL
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
NUPL or hRIP (human Rev interacting protein) was originally identified in a yeast two-hybrid screen for proteins that interact with the Rev protein of the human immunodeficiency virus type 1 (HIV-1). Structurally, hRIP contains an ARFGAP (ARF GTPase-activating protein) domain. hRIP was found to have homology with nucleoporins; thus it is also referred to as nucleoporin-like protein (NUPL) RIP. hRIP is essential to HIV-1 replication but not for cell viability, and it appears to be important for the export of HIV-1 RNA from the perinuclear region to the cytoplasm.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 512 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetNUPL
Antigen Species
Human
Gene ID3267
Gene Symbol
AGFG1
Gene Name
ArfGAP with FG repeats 1
Uniprot IDP52594
Protein Name
Arf-GAP domain and FG repeat-containing protein 1
Alternate Names
arf-GAP domain and FG repeat-containing protein 1 • arf-GAP domain and FG repeats-containing protein 1 • HIV-1 Rev-binding protein • HRB • hRIP, Rev interacting protein • nucleoporin-like protein RIP • RAB • Rab, Rev/Rex activation domain-binding protein • rev/Rex activation domain-binding protein • rev-interacting protein • RIP
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NUPL immobilized on solid support.
The epitope recognized by A301-792A maps to a region between residue 512 and 562 of human nucleoporin-like protein RIP using the numbering given in entry NP_004495.2 (GeneID 3267).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Western Blot (WB)1:2000 - 1:10,000
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-792A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-792A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-792AOpen
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