Bethyl Laboratories
Rabbit anti-ASF Antibody Affinity Purified
Primary
Catalog #A302-052A
TargetASF
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
Image 1 of 2
local_shipping
Order within 10 hours and 6 minutes for shipment by Tuesday, November 3
Product Description
Alternative splicing factor (ASF) is a member of the serine and arginine-rich family of proteins (SR proteins) which function as regulators of pre-mRNA splicing. ASF is a multifunctional RNA binding protein involved in pre-mRNA splicing, mRNA export, and mRNA translation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetASF
Antigen Species
Human
Gene ID6426
Gene Symbol
SRSF1
Gene Name
serine and arginine rich splicing factor 1
Uniprot IDQ07955
Protein Name
Serine/arginine-rich splicing factor 1
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ASF immobilized on solid support.
The epitope recognized by A302-052A maps to a region between residue 1 and 50 of human alternative splicing factor (splicing factor, arginine/serine-rich 1) using the numbering given in entry NP_008855.1 (GeneID 6426).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Western Blot (WB)1:2000 - 1:10,000
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-052A · Lot 3 (current lot)Open
Product DatasheetPDF · A302-052A · Lot 3 (current lot)Open
Safety Data SheetPDF · A302-052AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1