Bethyl Laboratories
Rabbit anti-hPrp3p Antibody Affinity Purified
Primary
Catalog #A302-073A
TargethPrp3p
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP
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Product Description
hPrp3p (human pre-mRNA splicing factor 3) was identified as a factor that associated with U4/U6 snRNPs which are components of the splicesome. hPrp3p is homologous to S. cerevisiae splicing factor Prp3p. Mutations in the PRPF3 gene are associated with retinitis pigmentosa-18. It has been determined that mutations in hPrp3p may alter the recognition motif for phosphorylation by casein kinase II (CKII) and that loss of phosphorylation may result in aberrant interactions within the U4/U6 snRNP complex.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 100 and 150
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargethPrp3p
Antigen Species
Human
Gene ID9129
Gene Symbol
PRPF3
Gene Name
pre-mRNA processing factor 3
Uniprot IDO43395
Protein Name
U4/U6 small nuclear ribonucleoprotein Prp3
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to hPrp3p immobilized on solid support.
The epitope recognized by A302-073A maps to a region between residue 100 and 150 of human U4/U6 small nuclear ribonucleoprotein Prp3 using the numbering given in entry NP_004689.1 (GeneID 9129).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-073A · Lot 2 (current lot)Open
Product DatasheetPDF · A302-073A · Lot 2 (current lot)Open
Safety Data SheetPDF · A302-073AOpen
Citations / Publications
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