Bethyl Laboratories
Rabbit anti-SRcyp Antibody Affinity Purified
Primary
Catalog #A302-075A
TargetSRcyp
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
SR cyclophilin (SRcyp) is also known as peptidyl-prolyl cis-trans isomerase G. Cyclophilins are peptidyl-prolyl isomerases (PPIases) that accelerate the folding of proteins by catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The function of cyclophilins is poorly understood, but their function has been linked to multiple cellular processes such as protein folding, trafficking, and chaperone activity. SRcyp is a member of the Moca family of nuclear cyclophilins and is phosphorylated in a cell cycle dependent manner. There is evidence that SRcyp may be involved in the regulation of gene expression and mRNA splicing.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 375 and 425
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSRcyp
Antigen Species
Human
Gene ID9360
Gene Symbol
PPIG
Gene Name
peptidylprolyl isomerase G
Uniprot IDQ13427
Protein Name
Peptidyl-prolyl cis-trans isomerase G
Alternate Names
CARS-cyclophilin • CARS-Cyp • CASP10 • Clk-associating RS-cyclophilin • cyclophilin G • CYP • peptidyl-prolyl cis-trans isomerase G • Peptidyl-prolyl isomerase G • peptidyl-prolyl isomerase G (cyclophilin G) • PPIase G • rotamase G • SCAF10 • SR-cyclophilin • SRCyp • SR-cyp • SR-related CTD-associated factor 10
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SRcyp immobilized on solid support.
The epitope recognized by A302-075A maps to a region between residue 375 and 325 of human SR-cyclophilin (Peptidyl-prolyl isomerase G) using the numbering given in entry NP_004783.2 (GeneID 9360).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-075A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-075A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-075AOpen
Citations / Publications
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