Bethyl Laboratories
Rabbit anti-SMG9 Antibody Affinity Purified
Primary
Catalog #A302-210A
TargetSMG9
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
SMG9 is a subunit of the SMG-1 complex. SMG-1 and Upf1 are part of a surveillance complex that participates in nonsense-mediated mRNA decay (NMD) to degrade mRNAs containing premature translation initiation codons (PTCs).
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 75 and 125
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSMG9
Antigen Species
Human
Gene ID56006
Gene Symbol
SMG9
Gene Name
SMG9, nonsense mediated mRNA decay factor
Uniprot IDQ9H0W8
Protein Name
Protein SMG9
Alternate Names
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SMG9 immobilized on solid support.
The epitope recognized by A302-210A maps to a region between residue 75 and 125 of human suppressor with morphogenetic effect on genitalia protein 9 using the numbering given in entry NP_061981.2 (GeneID 56006).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:2000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-210A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-210A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-210AOpen
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