Bethyl Laboratories
Rabbit anti-DHX30 Antibody Affinity Purified
Primary
Catalog #A302-218A
TargetDHX30
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
DHX30 (DEAH-box polypeptide 30) is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DHX30 has been implicated to be involved in the packaging of HIV-1 viral RNA and HIV-1 replication.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 375 and 425
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetDHX30
Antigen Species
Human
Gene ID22907
Gene Symbol
DHX30
Gene Name
DExH-box helicase 30
Uniprot IDQ7L2E3
Protein Name
Putative ATP-dependent RNA helicase DHX30
Alternate Names
ATP-dependent RNA helicase DHX30 • DDX30 • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 30 • DEAH (Asp-Glu-Ala-His) box helicase 30 • DEAH (Asp-Glu-Ala-His) box polypeptide 30 • DEAH box protein 30 • DEAH-box helicase 30 • NEDMIAL • putative ATP-dependent RNA helicase DHX30 • RETCOR • retina co-repressor
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DHX30 immobilized on solid support.
The epitope recognized by A302-218A maps to a region between residue 350 and 400 of human DEAH (Asp-Glu-Ala-His) box polypeptide 30 using the numbering given in entry NP_619520.1 (GeneID 22907).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-218A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-218A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-218AOpen
Citations / Publications
Services
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