Bethyl Laboratories
Rabbit anti-DDX43 Antibody Affinity Purified
Primary
Catalog #A302-328A
TargetDDX43
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
DDX43 is a DEAD box ATP-dependent RNA helicase. The DDX43 gene is also known as HAGE. It has been found to be expressed specifically in tumors.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 300 and 350
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetDDX43
Antigen Species
Human
Gene ID55510
Gene Symbol
DDX43
Gene Name
DEAD-box helicase 43
Uniprot IDQ9NXZ2
Protein Name
Probable ATP-dependent RNA helicase DDX43
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DDX43 immobilized on solid support.
The epitope recognized by A302-328A maps to a region between residue 275 and 325 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 43 using the numbering given in entry NP_061135.1 (GeneID 55510).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-328A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-328A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-328AOpen
Services
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