Bethyl Laboratories
Rabbit anti-NPM1 Antibody Affinity Purified
Primary
Catalog #A302-402A
TargetNPM1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationWB
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Product Description
Nucleophosmin (NPM1) is involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors TP53/p53 and ARF. It associates with nucleolar ribonucleoprotein structures and binds single-stranded nucleic acids. NPM1 binds ribosomes presumably to drive ribosome nuclear export. Chromosomal aberrations involving NPM1 have been identified in several types of leukemia [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P06748].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 100 and 150
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetNPM1
Antigen Species
Human
Gene ID4869
Gene Symbol
NPM1
Gene Name
nucleophosmin
Uniprot IDP06748
Protein Name
Nucleophosmin
Product Information
Applications
WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NPM1 immobilized on solid support.
The epitope recognized by A302-402A maps to a region between residue 100 and 150 of human nucleophosmin using the numbering given in entry NP_002511.1 (GeneID 4869).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-402A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-402A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-402AOpen
Citations / Publications
Services
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