Bethyl Laboratories
Rabbit anti-RIOK2 Antibody Affinity Purified
Primary
Catalog #A302-449A
TargetRIOK2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
RIO kinase 2 (RIOK2) is a member of the RIO family of atypical serine protein kinases first characterized in yeast. There are three RIO kinase subfamilies: RIO1, RIO2, and RIO3. RIOK1 and RIOK2 proteins are present in organisms from Archaea to humans. Studies of RIO kinases in yeast indicate that they play a role in ribosome biogenesis. Human RIOK2 has been shown to be a part of a late 40S pre-ribosomal particle and have influence on 40S maturation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 275 and 325
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRIOK2
Antigen Species
Human
Gene ID55781
Gene Symbol
RIOK2
Gene Name
RIO kinase 2
Uniprot IDQ9BVS4
Protein Name
Serine/threonine-protein kinase RIO2
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RIOK2 immobilized on solid support.
The epitope recognized by A302-449A maps to a region between residue 275 and 325 of human RIO kinase 2 using the numbering given in entry NP_060813.1 (GeneID 55781).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:5000
Documents
Product DatasheetPDF · A302-449A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-449A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-449AOpen
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