Bethyl Laboratories
Rabbit anti-SPF45 Antibody Affinity Purified
Primary
Catalog #A302-497A
TargetSPF45
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP
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Product Description
Splicing factor 45 (SPF45) is an RNA splicing factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. SPF45 is involved in the regulation of alternative splicing and the utilization of cryptic splice sites. It has been shown to promote the utilization of a cryptic splice site created by the beta-110 mutation in the human beta-thalassemia gene. The resulting frameshift leads to sickle cell anemia [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96125].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 25 and 75
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSPF45
Antigen Species
Human
Gene ID84991
Gene Symbol
RBM17
Gene Name
RNA binding motif protein 17
Uniprot IDQ96I25
Protein Name
Splicing factor 45
Alternate Names
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SPF45 immobilized on solid support.
The epitope recognized by A302-497A maps to a region between residue 25 and 75 of human splicing factor (45kD) using the numbering given in entry NP_116294.1 (GeneID 84991).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-497A · Lot 3 (current lot)Open
Product DatasheetPDF · A302-497A · Lot 3 (current lot)Open
Safety Data SheetPDF · A302-497AOpen
Citations / Publications
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