Bethyl Laboratories
Rabbit anti-RuvBL2 Antibody Affinity Purified
Primary
Catalog #A302-536A
TargetRuvBL2
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
RuvB-like 2 (RuvBL2) is the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this protein has both ATPase and DNA helicase activities [taken from NCBI Entrez Gene (GeneID: 9400)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRuvBL2
Antigen Species
Human
Gene ID10856
Gene Symbol
RUVBL2
Gene Name
RuvB like AAA ATPase 2
Uniprot IDQ9Y230
Protein Name
RuvB-like 2
Alternate Names
48 kDa TATA box-binding protein-interacting protein • 48 kDa TBP-interacting protein • 51 kDa erythrocyte cytosolic protein • CGI-46 • ECP51 • ECP-51 • erythrocyte cytosolic protein, 51-KD • INO80 complex subunit J • INO80J • repressing pontin 52 • REPTIN • Reptin 52 • reptin52 protein • RuvB (E coli homolog)-like 2 • ruvB-like 2 • RVB2 • TAP54-beta • TIH2 • TIP48 • TIP49B • TIP60-associated protein 54-beta
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RuvBL2 immobilized on solid support.
The epitope recognized by A302-536A maps to a region between residue 1 and 50 of human RuvB-like 2 using the numbering given in entry NP_006657.1 (GeneID 10856).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-536A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-536A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-536AOpen
Citations / Publications
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