Bethyl Laboratories
Rabbit anti-MCEF Antibody Affinity Purified
Primary
Catalog #A302-539A
TargetMCEF
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Major CDK9 elongation factor-associated protein (MCEF) is also known as AFF4 AF4/FMR2 family member 4. This family of proteins includes the AF4 proto-oncogene and FMR2 (fragile X E mental retardation syndrome) nuclear proteins. MCEF is a component of the cyclin-dependent kinase pair (CDK9/cyclin T1) complex. A chromosomal aberration involving AFF4 is found in acute lymphoblastic leukemia (ALL).
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1113 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMCEF
Antigen Species
Human
Gene ID27125
Gene Symbol
AFF4
Gene Name
AF4/FMR2 family member 4
Uniprot IDQ9UHB7
Protein Name
AF4/FMR2 family member 4
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MCEF immobilized on solid support.
The epitope recognized by A302-539A maps to a region between residue 1113 and 1163 of human major CDK9 elongation factor-associated protein of human using the numbering given in entry NP_055238.1 (GeneID 27125).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-539A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-539A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-539AOpen
Citations / Publications
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