Bethyl Laboratories
Rabbit anti-RACK1 Antibody Affinity Purified
Primary
Catalog #A302-545A
TargetRACK1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
RACK1 (receptor of activated protein kinase C1) was originally identified as a factor that could bind the activated form of protein kinase C. It contains seven WD40 motifs that interact with various signaling molecules.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRACK1
Antigen Species
Human
Gene ID10399
Gene Symbol
RACK1
Gene Name
receptor for activated C kinase 1
Uniprot IDP63244
Protein Name
Receptor of activated protein C kinase 1
Alternate Names
cell proliferation-inducing gene 21 protein • GNB2L1 • Gnb2-rs1 • guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 • guanine nucleotide binding protein beta polypeptide 2-like 1 • guanine nucleotide-binding protein subunit beta-2-like 1 • guanine nucleotide-binding protein subunit beta-like protein 12.3 • H12.3 • HLC-7 • human lung cancer oncogene 7 protein • lung cancer oncogene 7 • PIG21 • proliferation-inducing gene 21 • protein homologous to chicken B complex protein, guanine nucleotide binding • Receptor for activated C kinase • receptor of activated protein C kinase 1 • receptor of activated protein kinase C 1 • small ribosomal subunit protein RACK1
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RACK1 immobilized on solid support.
The epitope recognized by A302-545A maps to a region between residue 1 and 50 of human receptor of activated protein kinase C 1 using the numbering given in entry NP_006089.1 (GeneID 10399).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Western Blot (WB)1:10,000 - 1:25,000
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-545A · Lot 3 (current lot)Open
Product DatasheetPDF · A302-545A · Lot 3 (current lot)Open
Safety Data SheetPDF · A302-545AOpen
Citations / Publications
Services
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