Bethyl Laboratories
Rabbit anti-eIF2B5 Antibody Affinity Purified
Primary
Catalog #A302-557A
TargeteIF2B5
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
Image 1 of 2
local_shipping
Order within 9 hours and 33 minutes for shipment by today
Product Description
eIF2B5 is one of five subunits of the eukaryotic translation initiation factor 2B (eIF2B). eIF2B5 functions as a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in the EIF2B5 gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter (VWM) [taken from NCBI Entrez Gene (GeneID: 8893)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 550 and 600
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargeteIF2B5
Antigen Species
Human
Gene ID8893
Gene Symbol
EIF2B5
Gene Name
eukaryotic translation initiation factor 2B subunit epsilon
Uniprot IDQ13144
Protein Name
Translation initiation factor eIF-2B subunit epsilon
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to eIF2B5 immobilized on solid support.
The epitope recognized by A302-557A maps to a region between residue 550 and 600 of human eukaryotic translation initiation factor 2B, subunit 5 epsilon using the numbering given in entry NP_003898.2 (GeneID 8893).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-557A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-557A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-557AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1