Bethyl Laboratories
Rabbit anti-Ku80 Antibody Affinity Purified
Primary
Catalog #A302-627A
TargetKu80
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
Ku80 is the 80 kDa subunit of the lupus Ku autoantigen. The lupus Ku autoantigen was identified in individuals with systemic lupus erythematosus (SLE) and related disorders. The Ku antigen is a heterodimer of Ku70 and Ku80. The Ku70/Ku80 complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P13010].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 150 and 200
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetKu80
Antigen Species
Human
Gene ID7520
Gene Symbol
XRCC5
Gene Name
X-ray repair cross complementing 5
Uniprot IDP13010
Protein Name
X-ray repair cross-complementing protein 5
Alternate Names
86 kDa subunit of Ku antigen • ATP-dependent DNA helicase 2 subunit 2 • ATP-dependent DNA helicase II 80 kDa subunit • CTC box-binding factor 85 kDa subunit • CTC85 • CTCBF • DNA repair protein XRCC5 • KARP1 • KARP-1 • Ku autoantigen, 80kDa • KU80 • Ku86 • Ku86 autoantigen related protein 1 • KUB2 • lupus Ku autoantigen protein p86 • NFIV • nuclear factor IV • thyroid-lupus autoantigen • TLAA • X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) • X-ray repair cross-complementing protein 5
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Ku80 immobilized on solid support.
The epitope recognized by A302-627A maps to a region between residue 150 and 200 of human Lupus Ku autoantigen protein p86 using the numbering given in entry NP_066964.1 (GeneID 7520).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-627A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-627A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-627AOpen
Citations / Publications
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