Bethyl Laboratories
Rabbit anti-Exonuclease 1 Antibody Affinity Purified
Primary
Catalog #A302-640A
TargetExonuclease 1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Exonuclease 1 is a 5'-3' double-stranded DNA exonuclease which may also possess a cryptic 3'-5' double-stranded DNA exonuclease activity. Exonuclease 1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. It also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Exonuclease 1 is required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes and is essential for male and female meiosis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9UQ84].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 796 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetExonuclease 1
Antigen Species
Human
Gene ID9156
Gene Symbol
EXO1
Gene Name
exonuclease 1
Uniprot IDQ9UQ84
Protein Name
Exonuclease 1
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Exonuclease 1 immobilized on solid support.
The epitope recognized by A302-640A maps to a region between residue 796 and 846 of human exonuclease 1 using the numbering given in entry NP_006018.3 (GeneID 9156).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-640A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-640A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-640AOpen
Citations / Publications
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