Rabbit anti-SMG5 Antibody Affinity Purified

Specifications

Production & Epitope

Antibody was affinity purified using an epitope specific to SMG5 immobilized on solid support.

The epitope recognized by A302-804A maps to a region between residue 400 and 450 of human Suppressor with Morphogenetic Effects on Genitalia 5 using the numbering given in entry NP_056142.2 (GeneID 23381).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.