Bethyl Laboratories
Rabbit anti-C1QBP Antibody Affinity Purified
Primary
Catalog #A302-862A
TargetC1QBP
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
C1qBP (complement component 1q subcomponent-binding protein) is known to bind to the globular heads of the human complement component, C1q, and inhibit C1 activation. This protein has also been identified as the p32 subunit of pre-mRNA splicing factor SF2, as well as a hyaluronic acid-binding protein (HABP1) [taken from NCBI Entrez Gene (Gene ID: 10084)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 150 and 200
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetC1QBP
Antigen Species
Human
Gene ID708
Gene Symbol
C1QBP
Gene Name
complement C1q binding protein
Uniprot IDQ07021
Protein Name
Complement component 1 Q subcomponent-binding protein, mitochondrial
Alternate Names
ASF/SF2-associated protein p32 • C1q globular domain-binding protein • C1qBP • complement component 1 Q subcomponent-binding protein, mitochondrial • complement component 1, q subcomponent binding protein • COXPD33 • GC1QBP • gC1qR • gC1Q-R • gC1q-R protein • glycoprotein gC1qBP • HABP1 • hyaluronan-binding protein 1 • mitochondrial matrix protein p32 • p32 • p33 • SF2AP32 • SF2p32 • splicing factor SF2-associated protein
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to C1QBP immobilized on solid support.
The epitope recognized by A302-862A maps to a region between residue 150 and 200 of human Complement component 1, q Subcomponent Binding Protein using the numbering given in entry NP_001203.1 (GeneID 708).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-862A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-862A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-862AOpen
Citations / Publications
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