Bethyl Laboratories
Rabbit anti-DDX47 Antibody Affinity Purified
Primary
Catalog #A302-976A
TargetDDX47
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
DDX47 (DEAD box polypeptide 47) is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX47 can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetDDX47
Antigen Species
Human
Gene ID51202
Gene Symbol
DDX47
Gene Name
DEAD-box helicase 47
Uniprot IDQ9H0S4
Protein Name
Probable ATP-dependent RNA helicase DDX47
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DDX47 immobilized on solid support.
The epitope recognized by A302-976A maps to a region between residue 1 and 50 of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 47 using the numbering given in entry NP_057439.2 (GeneID 51202).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-976A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-976A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-976AOpen
Citations / Publications
Services
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