Bethyl Laboratories
Rabbit anti-DDX56 Antibody Affinity Purified
Primary
Catalog #A302-979A
TargetDDX56
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
DDX56 (DEAD box polypeptide 56) is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX56 is propososed to be involved in ribosome biosynthesis and the assembly of the large (60S) ribosomal subunit.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 497 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetDDX56
Antigen Species
Human
Gene ID54606
Gene Symbol
DDX56
Gene Name
DEAD-box helicase 56
Uniprot IDQ9NY93
Protein Name
Probable ATP-dependent RNA helicase DDX56
Alternate Names
61-kd nucleolar helicase • ATP-dependent 61 kDa nucleolar RNA helicase • DDX21 • DDX26 • DEAD (Asp-Glu-Ala-Asp) box helicase 56 • DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 • DEAD box protein 21 • DEAD box protein 56 • DEAD-box RNA helicase • NOH61 • nucleolar helicase of 61 kDa • probable ATP-dependent RNA helicase DDX56 • putative nucleolar RNA helicase
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DDX56 immobilized on solid support.
The epitope recognized by A302-979A maps to a region between residue 497 and 547 of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 56 using the numbering given in entry NP_061955.1 (GeneID 54606).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-979A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-979A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-979AOpen
Citations / Publications
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