Bethyl Laboratories
Rabbit anti-DDX55 Antibody Affinity Purified
Primary
Catalog #A303-027A
TargetDDX55
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
DDX55 is a member of of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.DDX55 is a probable ATP-binding RNA helicase [taken from NCBI Entrez Gene (Gene ID: 57696)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 200 and 250
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetDDX55
Antigen Species
Human
Gene ID57696
Gene Symbol
DDX55
Gene Name
DEAD-box helicase 55
Uniprot IDQ8NHQ9
Protein Name
ATP-dependent RNA helicase DDX55
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DDX55 immobilized on solid support.
The epitope recognized by A303-027A maps to a region between residue 175 and 225 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 55 using the numbering given in entry NP_065987.1 (GeneID 57696).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-027A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-027A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-027AOpen
Citations / Publications
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