Bethyl Laboratories
Rabbit anti-SSRP1 Antibody Affinity Purified
Primary
Catalog #A303-068A
TargetSSRP1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
Structure specific recognition protein 1 (SSRP1) is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. SSRP1 also functions as a co-activator of the transcriptional activator p63 [taken from NCBI Entrez Gene (Gene ID: 6749].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 150 and 200
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSSRP1
Antigen Species
Human
Gene ID6749
Gene Symbol
SSRP1
Gene Name
structure specific recognition protein 1
Uniprot IDQ08945
Protein Name
FACT complex subunit SSRP1
Alternate Names
chromatin-specific transcription elongation factor 80 kDa subunit • cisplatin-DNA SSRP • facilitates chromatin remodeling 80 kDa subunit • facilitates chromatin transcription complex 80 kDa subunit • facilitates chromatin transcription complex subunit SSRP1 • FACT • FACT 80 kDa subunit • FACT complex subunit SSRP1 • FACT80 • FACTp80 • high mobility group box • hSSRP1 • recombination signal sequence recognition protein 1 • Structure-specific recognition protein 1 • T160
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SSRP1 immobilized on solid support.
The epitope recognized by A303-068A maps to a region between residue 150 and 200 of human Structure Specific Recognition Protein 1 using the numbering given in entry NP_003137.1 (GeneID 6749).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-068A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-068A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-068AOpen
Citations / Publications
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