Bethyl Laboratories
Rabbit anti-TDP43 Antibody Affinity Purified
Primary
Catalog #A303-223A
TargetTDP43
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Tar DNA-binding protein 43 (TDP43) is a DNA and RNA-binding protein which regulates transcription and splicing. It is involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. TDP43 may also be involved in microRNA biogenesis, apoptosis and cell division. TDP43 can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Also, TDP43 stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q13148].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 175 and 225
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetTDP43
Antigen Species
Human
Gene ID23435
Gene Symbol
TARDBP
Gene Name
TAR DNA binding protein
Uniprot IDQ13148
Protein Name
TAR DNA-binding protein 43
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to TDP43 immobilized on solid support.
The epitope recognized by A303-223A maps to a region between residue 150 and 200 of human TAR DNA-Binding Protein 43 using the numbering given in entry NP_031401.1 (GeneID 23435).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:200 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-223A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-223A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-223AOpen
Citations / Publications
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