Bethyl Laboratories
Rabbit anti-CENP-I Antibody Affinity Purified
Primary
Catalog #A303-374A
TargetCENP-I
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Centromere protein I (CENP-I) is a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. CENP-I may be involved in incorporation of newly synthesized CENP-A into centromeres via its interaction with the CENPA-NAC complex. CENP-I is required for the localization of CENP-F, MAD1L1 and MAD2 (MAD2L1 or MAD2L2) to kinetochores. CENP-A is involved in the response of gonadal tissues to follicle-stimulating hormone [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q92674].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 50 and 100
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCENP-I
Antigen Species
Human
Gene ID2491
Gene Symbol
CENPI
Gene Name
centromere protein I
Uniprot IDQ92674
Protein Name
Centromere protein I
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CENP-I immobilized on solid support.
The epitope recognized by A303-374A maps to a region between residue 50 and 100 of human Centromere Protein I using the numbering given in entry NP_006724.2 (GeneID 2491).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-374A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-374A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-374AOpen
Citations / Publications
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