Bethyl Laboratories
Rabbit anti-SNRNP200 Antibody Affinity Purified
Primary
Catalog #A303-453A
TargetSNRNP200
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
Image 1 of 2
local_shipping
Order within 6 hours and 15 minutes for shipment by today
Product Description
U5 small nuclear ribonucleoprotien 200kDa (SNRNP200) is one of nine U5 snRNP-specific proteins. SNRNP200 belongs to the DEXH-box family of putative RNA helicases. It is a core component of U4/U6-U5 snRNPs and appears to catalyze an ATP-dependent unwinding of U4/U6 RNA duplices. Mutations in the SNRNP200 gene cause autosomal-dominant retinitis pigmentosa [taken from NCBI Entrez Gene (Gene ID: 23020)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSNRNP200
Antigen Species
Human
Gene ID23020
Gene Symbol
SNRNP200
Gene Name
small nuclear ribonucleoprotein U5 subunit 200
Uniprot IDO75643
Protein Name
U5 small nuclear ribonucleoprotein 200 kDa helicase
Alternate Names
activating signal cointegrator 1 complex subunit 3-like 1 • ASCC3L1 • bad response to refrigeration 2 homolog • BRR2 • BRR2 homolog • HELIC2 • RP33 • small nuclear ribonucleoprotein 200kDa (U5) • small nuclear ribonucleoprotein, U5 200kDa subunit • U5 small nuclear ribonucleoprotein 200 kDa helicase • U5 snRNP-specific 200 kDa protein • U5-200KD
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SNRNP200 immobilized on solid support.
The epitope recognized by A303-453A maps to a region between residue 1 and 50 of human Small Nuclear Ribonucleoprotein 200kDa using the numbering given in entry NP_054733.2 (GeneID 23020).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A303-453A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-453A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-453AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1