Bethyl Laboratories
Rabbit anti-CCT3 Antibody Affinity Purified
Primary
Catalog #A303-459A
TargetCCT3
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
CCT3 is also known as T complex protein 1 subunit gamma (TCP1-gamma) and is a molecular chaperone that assists in the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT3 may play a role in the assembly of BBSome, a complex involved in ciliogenesis and regulating the transport of vesicles to the cilia. CCT3 is known to play a role, in vitro, in the folding of actin and tubulin [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P49368].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 495 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetCCT3
Antigen Species
Human
Gene ID7203
Gene Symbol
CCT3
Gene Name
chaperonin containing TCP1 subunit 3
Uniprot IDP49368
Protein Name
T-complex protein 1 subunit gamma
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CCT3 immobilized on solid support.
The epitope recognized by A303-459A maps to a region between residue 495 and 545 of human Chaperonin containing TCP1, Subunit 3 using the numbering given in entry NP_005989.3 (GeneID 7203).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-459A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-459A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-459AOpen
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