Bethyl Laboratories
Rabbit anti-CCT2 Antibody Affinity Purified
Primary
Catalog #A303-478A
TargetCCT2
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Chaperonin containing TCP1, subunit 2 (beta) (CCT2) is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin [taken from NCBI Entrez Gene (Gene ID: 10576)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 325 and 375
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetCCT2
Antigen Species
Human
Gene ID10576
Gene Symbol
CCT2
Gene Name
chaperonin containing TCP1 subunit 2
Uniprot IDP78371
Protein Name
T-complex protein 1 subunit beta
Alternate Names
99D8.1 • CCTB • CCT-beta • chaperonin containing t-complex polypeptide 1, beta subunit • chaperonin containing t-complex polypeptide 1, subunit 2 • chaperonin containing TCP1, subunit 2 (beta) • epididymis secretory sperm binding protein Li 100n • HEL-S-100n • PRO1633 • T-complex protein 1 subunit beta • T-complex protein 1, beta subunit • TCP-1-beta
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CCT2 immobilized on solid support.
The epitope recognized by A303-478A maps to a region between residue 325 and 375 of human Chaperonin Containing TCP1, Subunit 2 using the numbering given in entry NP_006422.1 (GeneID 10576).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-478A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-478A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-478AOpen
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