Bethyl Laboratories
Rabbit anti-CDK9 Antibody Affinity Purified
Primary
Catalog #A303-493A
TargetCDK9
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Cyclin-dependent kinase 9 (CDK9) is a member of the CDK family of kinases and a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. CDK9 forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 322 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetCDK9
Antigen Species
Human
Gene ID1025
Gene Symbol
CDK9
Gene Name
cyclin dependent kinase 9
Uniprot IDP50750
Protein Name
Cyclin-dependent kinase 9
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CDK9 immobilized on solid support.
The epitope recognized by A303-493A maps to a region between residue 322 and 372 of human Cyclin-Dependent Kinase 9 using the numbering given in entry NP_001252.1 (GeneID 1025).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-493A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-493A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-493AOpen
Citations / Publications
Services
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