Bethyl Laboratories
Rabbit anti-DCP1A Antibody Affinity Purified
Primary
Catalog #A303-590A
TargetDCP1A
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
mRNA-decapping enzyme 1A (DCP1A) is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. DCP1A removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. DCP1A also contributes to the transactivation of target genes after stimulation by TGFB1 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9NPI6].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 225 and 275
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetDCP1A
Antigen Species
Human
Gene ID55802
Gene Symbol
DCP1A
Gene Name
decapping mRNA 1A
Uniprot IDQ9NPI6
Protein Name
mRNA-decapping enzyme 1A
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DCP1A immobilized on solid support.
The epitope recognized by A303-590A maps to a region between residue 225 and 275 of human DCP1 Decapping Enzyme Homolog A using the numbering given in entry NP_060873.4 (GeneID 55802).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-590A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-590A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-590AOpen
Citations / Publications
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