Bethyl Laboratories
Rabbit anti-Gamma-Adducin Antibody Affinity Purified
Primary
Catalog #A303-715A
TargetGamma-Adducin
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Gamma-adducin is a member of the adducin family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Originally purified from human erythrocytes, adducin was found to be a heterodimer of adducins alpha and beta. Heterodimers consisting of alpha and gamma subunits have also been described [taken from NCBI Entrez Gene (Gene ID: 120)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 600 and 650
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetGamma-Adducin
Antigen Species
Human
Gene ID120
Gene Symbol
ADD3
Gene Name
adducin 3
Uniprot IDQ9UEY8
Protein Name
Gamma-adducin
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Gamma-Adducin immobilized on solid support.
The epitope recognized by A303-715A maps to a region between residue 600 and 650 of human Adducin 3 (gamma) using the numbering given in entry NP_058432.1 (GeneID 120).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-715A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-715A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-715AOpen
Services
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