Bethyl Laboratories
Rabbit anti-MPP11 Antibody Affinity Purified
Primary
Catalog #A303-721A
TargetMPP11
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
M-phase phosphoprotein 11 is a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. MPP11 is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. MPP11 was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Additionally, chromosomal aberrations involving the DNAJC2 gene are associated with primary head and neck squamous cell tumors [taken from NCBI Entrez Gene (Gene ID: 27000)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 500 and 550
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMPP11
Antigen Species
Human
Gene ID27000
Gene Symbol
DNAJC2
Gene Name
DnaJ heat shock protein family (Hsp40) member C2
Uniprot IDQ99543
Protein Name
DnaJ homolog subfamily C member 2
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MPP11 immobilized on solid support.
The epitope recognized by A303-721A maps to a region between residue 500 and 550 of human DnaJ (Hsp40) Homolog, Subfamily C, Member 2 using the numbering given in entry NP_055192.1 (GeneID 27000).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-721A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-721A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-721AOpen
Citations / Publications
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