Bethyl Laboratories
Rabbit anti-CAPRIN1 Antibody Affinity Purified
Primary
Catalog #A303-882A
TargetCAPRIN1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
CAPRIN1 (cell cycle-associated protein 1) may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q14444].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 550 and 600
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCAPRIN1
Antigen Species
Human
Gene ID4076
Gene Symbol
CAPRIN1
Gene Name
cell cycle associated protein 1
Uniprot IDQ14444
Protein Name
Caprin-1
Alternate Names
activation/proliferation-associated protein 1 • caprin 1 • caprin-1 • Cell cycle-associated protein 1 • cytoplasmic activation- and proliferation-associated protein 1 • cytoplasmic activation/proliferation-associated protein-1 • GPI-anchored membrane protein 1 • GPI-anchored protein p137 • GPIAP1 • GPIP137 • GPI-p137 • GRIP137 • M11S1 • membrane component chromosome 11 surface marker 1 • p137GPI • RNA granule protein 105 • RNG105
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CAPRIN1 immobilized on solid support.
The epitope recognized by A303-882A maps to a region between residue 550 and 600 of human Cell Cycle Associated Protein 1 using the numbering given in entry NP_005889.3 (GeneID 4076).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Western Blot (WB)1:10,000 - 1:25,000
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A303-882A · Lot 2 (current lot)Open
Product DatasheetPDF · A303-882A · Lot 2 (current lot)Open
Safety Data SheetPDF · A303-882AOpen
Citations / Publications
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