Bethyl Laboratories
Rabbit anti-EXOSC9 Antibody Affinity Purified
Primary
Catalog #A303-888A
TargetEXOSC9
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
Image 1 of 3
local_shipping
Order within 4 hours and 50 minutes for shipment by today
Product Description
EXOSC9 is a non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm [taken from the Universal Protein Resource (UniProt) http://www.uniprot.org/uniprot/Q06265.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 389 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetEXOSC9
Antigen Species
Human
Gene ID5393
Gene Symbol
EXOSC9
Gene Name
exosome component 9
Uniprot IDQ06265
Protein Name
Exosome complex component RRP45
Alternate Names
autoantigen PM/Scl 1 • exosome complex component RRP45 • exosome complex exonuclease RRP45 • Exosome component 9 • p5 • p6 • P75 polymyositis-scleroderma overlap syndrome associated autoantigen • P75 polymyositis-scleroderma overlap syndrome-associated autoantigen • PCH1D • PM/Scl-75 • PMSCL autoantigen, 75kD • PMSCL1 • Polymyositis/scleroderma autoantigen 1 • polymyositis/scleroderma autoantigen 1, 75kDa • Polymyositis/scleroderma autoantigen 75 kDa • RRP45 • Rrp45p
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to EXOSC9 immobilized on solid support.
The epitope recognized by A303-888A maps to a region between residue 389 and 439 of human Exosome Component 9 using the numbering given in entry NP_005024.2 (GeneID 5393).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-888A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-888A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-888AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1