Bethyl Laboratories
Rabbit anti-PAPD1 Antibody Affinity Purified
Primary
Catalog #A303-904A
TargetPAPD1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
PAPD1 (mitochondrial poly(A) polymerase) is a polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. PAPD1 can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). It plays a role in replication-dependent histone mRNA degradation. It may be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated and might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA [taken from NCBI Entrez Gene (Gene ID: 55149)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 532 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPAPD1
Antigen Species
Human
Gene ID55149
Gene Symbol
MTPAP
Gene Name
mitochondrial poly(A) polymerase
Uniprot IDQ9NVV4
Protein Name
Poly(A) RNA polymerase, mitochondrial
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PAPD1 immobilized on solid support.
The epitope recognized by A303-904A maps to a region between residue 532 and 582 of human PAP-Associated Domain-Containing Protein 1 using the numbering given in entry NP_060579.3 (GeneID 55149).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-904A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-904A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-904AOpen
Citations / Publications
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